What is the use of 3 MM Whatman paper in biological assays ?

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Greg Cote

We use 3MM paper for polysaccharide synthase assays, in which the radioactively labeled glycans are precipitated in the paper fibers using ethanol or methanol. Acetone would probably accomplish the same thing.

J. Hemberger

TCA will precipitate most high molecular polymers, especially proteins which will then be recovered on 3 MM Whatman paper. We have used this method with radioactive labelling to study for instance protein-DNA interactions. The Whatman paper will serve to separate precipitated material from soluble substances.

Manesh Nautiyal

Thanks for useful comments.