Manesh Nautiyal

7 Questions 9 Answers 0 Followers

Questions related from Manesh Nautiyal

Please specify the sources for buying Carbon 14 labelled amino acids ?

I want to perform some radio labeled transfer assays using Carbon 14 labeled amino acids. I know some sources e.g., PerkinElmer, Moravek for radioactive chemicals. If you know any other please let...

03 December 2018 9,799 4 View

How to extract the cytoplasmic content from human cells ?

Dear all, I want only cytoplasmic content from the human cells to test my compounds. Does anyone knows how to do it or can share the protocol for doing it ?

30 June 2018 9,203 2 View

How to perform the statistical F-test analyses to find out which equation (e.g. michaelis-menton, or morrison) need to be used for fitting of data ?

For the analysis of in vitro data I am using GraphPad software and utilizing various equation (logarithms) to fit data. How can I know that which equation should be used and it is correct ?

14 May 2018 4,530 2 View

Does anyone observed formation of Triethylamine-dichloromethane salt ?

Has anyone observed the formation of dichlormethane-triethylamine salt, while performing the purification in silica column with DCM:methanol:triethylamine (95:4:1) system as mobile phase ? If yes...

16 February 2018 8,498 6 View

What does "S" stand for in s30 cell extract ?

s30 cell extract is the most common medium to study the protein functionality of the cell.

17 August 2015 788 5 View

What is the use of 3 MM Whatman paper in biological assays ?

What are the things (amino acid, peptide, proteins or what ) which can be precipitated in that paper with the use of TCA and acetone washing?

03 August 2015 3,048 3 View

How can I determine which Column should be used in HPLC to purify compounds? Is there is any specific guidelines mentioned?

I want to remove amino acid fragments impurities from my compounds containing sulphonamide, phosphate or carbamates groups. Thanks in advance for any suggestion.

22 May 2015 855 4 View