i want to do RNA extraction on a tissue sample that has undergone paraffinization. After the deparaffinization using melting i did rna extraction and the result is a very low concentrate of rna. What could have gone wrong in the first procedure?
Does anyone know documents for national inventories of agroforestry systems, to quickly collect data both remotely, with satellite data, and in the field, on representative areas (no more than...
21 March 2023 7,396 1 View
Thank you for checking this post out! I'll use the following example to discuss the challenge I'm facing: My logistic-exposure model asks whether a study species' nest success (1/0) can be...
25 April 2022 6,923 7 View
For instance I have noted the use of PICs (Pacific Island Countries), PICTs (Pacific Island Countries and Territories), PSIDS (Pacific Small Island Developing States). My research on marine...
28 September 2021 9,799 3 View
There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...
18 February 2021 9,564 5 View
I have tried to lyse my A549 cells for polysome profiling. I collect 5 150mm plates about 80-90% and have tried many conditions, but I can't get more than 5 OD260 units. With the same protocols I...
03 November 2019 3,821 0 View
Which are the main agroforestry strategies for carbon sequestration? Are the crops yield and the farmers income affected by these strategies?
10 June 2019 326 21 View
Dear friends and colleagues, Are you aware of existing or developing infrastructure works, under which two or more renewable energy technologies are coordinated towards the co-generation of green...
27 May 2019 6,217 15 View
I am currently working on premature infant nose breathing and I will need a premature infant nose throat-model to perform respiratory flow rate measurements. Thus, I would be very grateful if...
20 May 2019 974 0 View
Which are the main Carbon Capture and Storage (CCS) strategies? Are there technological constraints upon them -such as technological immaturity issues- or regional specifications (being...
19 May 2019 3,239 4 View
I am last standing in closing lab far from main campus. The few others in building don't know how to work with frozen tissues this large. I need to get images fast because time is running out....
11 April 2018 457 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View