I am last standing in closing lab far from main campus. The few others in building don't know how to work with frozen tissues this large. I need to get images fast because time is running out. Brains have been perfused and placed in 30% sucrose and frozen in cryoprotectant media at -80. Sections will be 30um thick. C57Bl6 mice brains with GBM, need to find adequate stain to use to get clear images so i can use imageJ. Need this to finish up PhD work before lab shuts down and i lose access. I have some cresyl violet (expired but probably still good) and have access to H&E in another lab-will either of these work to define tumour edges enough to get clear images? what do i have to do to get these stable at room temp?
Hi Paris,
if you do not have anything else in the lab, so the best possibility for you to see the tumor edges in the brain is to stain some sections with H&E, simply because it will be better to see accumulation of cells in a cluster which might be a preneoplastic foci. Cresyl violet could be not so helpful.
Good luck to have something
Imam
Paris,
H&E is a very easy, efficient, and durable method. You should be able to get the staining done based on your details. Please specify better what further assistance do you need exactly.
Good luck
Boaz