How can I avoid any possible chances of getting algae in my rice hydroponics system?

I use light protected jars and big falcon tubes to grow my plants. Usually, I don't get algal contamination when growing plants for 2-3 weeks. But, sometimes I get algal growth in my samples. It...

05 June 2019 9,333 5 View

My T-DNA line, miRNA lines and over-expression lines showing similar phenotype under stress?

I am working on characterizing the function of a gene under stress. The T-DNA lines were obtained from SALK. The miRNA was prepared in lab and it shows very insignificant expression of my gene....

01 February 2017 9,367 4 View

What are the features of DH-5 alpha plasmids and mutation target is genome or plasmid?

03 April 2015 4,727 5 View

What is the shelf life of lab prepared Krebs-Ringer Bicarbonate Buffer?

I have prepared KRB buffer in lab for animal cell culture experiment. Can anyone let me know the shelf life of this KRB solution. I have stored this buffer at 4 degree celcius.

01 February 2015 7,827 2 View

Can a non MBBS undergraduate student join a post graduate or PHD medical/medicine course at a reputed university?

If a student from Biotechnology undergraduate field want to pursue his masters and/or PHD in medicine, is he eligible ? If yes, will he be able to cope up with the curriculum or syllabus (MBBS...

04 May 2014 3,224 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View