What is the thickness of adherent HEK293, HEK293T cells growing on cell culture plates?
The commonly cited value of average cell size (diameter) for spherical HEK293 cells is 11-15 μm (https://www.beckman.com/resources/product-applications/lead-optimization/cell-line-development/human-embryonic-kidney-293).
But what is their thickness when they are flattened while growing as adherent culture on TC-treated cell culture plates?
Dear Dr. Michael Koksharov,
The thickness of HEK293 cells does not change significantly upon flattening because they are known for their ability to form a flattened, elongated morphology when they adhere to the substratum, while maintaining a relatively consistent thickness.
When the cell changes shape, its overall thickness, or depth, remains relatively constant. The cell doesn't compress into a thinner layer when it spreads. The transition is primarily about the cell spreading and flattening rather than becoming thinner.
There is an increase in the cell's surface area as it flattens, not a decrease in thickness.
Best,
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