What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View

Do you consider that rose bengal chloramphenicol agar is suitable to isolate and count fungus?

Someone knows about a specific law or regulation which mentions the use of the rose bengal chloramphenicol agar is suitable to isolate and count fungi. Perhaps, someone has done a research about...

07 January 2021 5,325 5 View

No title

07 January 2021 9,052 1 View

Regression analysis on small but complete dataset (perfect representation of reality)?

I have a repeated measures design with about 16 cases and 5-6 points of measuring. Sometimes, 1-4 full cases or some points of measure are missing. (The measures are 20 numerical and categorical...

20 December 2020 9,004 7 View

Approval for MnFe2O4 for industrial wastewater treatment?

I am using MnFe2O4 NPs as adsorbent for CrVI for wastewater treatment following literatures as it is one of the best, but I have a challenge of releasing Mn+2 in the environment. A lot of...

19 November 2020 3,404 4 View

FISH probe synthesis with PCR?

Hello, I have some troubles with the synthesis of a DNA probe for FISH in paraffin tissues, we decide to synthesize it by PCR with labelled dUTP, but we haven't had any results, I tried chaging...

08 September 2020 183 3 View

HAP1 cell transfection with RNP complexes?

Hi, Does anyone have experience with transfecting HAP1 cells for genome editing with Cas9/gRNA complexes? Or are the HAP1 cells difficult to transfect? Thanks.

27 August 2020 6,028 1 View

Psychologically, How can you support a Covid-19 patient ?

After being affected with that viruse , most of the patients feel depressed and sad . How can we , psychologically, encourage them ?

26 August 2020 6,178 93 View

How does COVID-19 affect the Stakeholder Engagement of YOUR EU Energy OR Marine Projects?

Dear researchers, The COVID-19 pandemic has large societal and economic impacts, from which also different stakeholders involved in EU H2020 energy projects are affected. With this survey, we...

17 June 2020 4,542 4 View

MIKE 21 Illegal Storage Access?

Has anyone ever received the error 'Illegal storage access Abnormal completion' from MIKE? I am using the Data Extractor FM and at certain times this error comes up. Once I receive this error I...

24 March 2020 2,518 3 View

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03 March 2021 5,360 2 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

HiPSC positive for GLAST in FACS - why?

Hey, I'm working on hiPSC differentiation into astrocytes. I stained my cells (hiPSC stage) with a GLAST (ASCA1) antibody for FACS and the cells showed a strong positive signal. As far as I know,...

09 February 2021 3,383 4 View

How to best prepare cells for FACS from live tissue?

Hi everyone, I'm currently trying to perform single cell RNA seq on a small (

24 January 2021 9,804 2 View

Can zinc based fixatives be used after enzymatic dissociation of neural tissue?

I am working on a FACS experiment with mouse neural tissue. I want to preserve all cell types as much as possible and the zinc fixation papers I have read use mechanical dissociation which can...

21 January 2021 4,430 1 View

Does anybody know about literature on protest and post-conflict processes/societies? What would you recommend?

I am trying to start a project on the subject and your help would be appreciated

18 January 2021 2,190 6 View

Do polymers with different chain lengths have UV-Vis absorption peaks at different wavelengths?

I have a sample of mixtures containing polystyrene microspheres (PS-MS) with 3 different diameter sizes (bought from thermo), they all contain a green fluorescent dye inside the polymer matrix /...

06 January 2021 2,218 5 View

Is there any standard rubric in writing?

i am currently conducting my action research using color-coded cards(Colored cards )to the creative writing skills of my learners,do you have any recommendation for a standard rubric for my pre...

03 January 2021 2,862 2 View

Why pink coloured or light red onion have more storage life than white and dark red one and smaller size one to has more than bigger one?

It is generally seen light coloured varieties has more storage life.what's relation with colour and size with storage life.

13 December 2020 634 18 View