What is the suitable concentration of phosphatse and phosphorylase inhibitors which I have to use in my lysis buffer?

23 November 2013 1 2K Report

I need to prepare 8 M urea as lysis buffer to extract the proteins from cancer cell lines. Therefore I 'd like to know what suitable amount of cocktail inhibitors shall I use?

Meysam Kashiri

Usually 1/1000 phosphatase inhibitor,

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