What is the subcellular localization of procaspase-7 and active caspase-7?

29 June 2018 1 3K Report

There are reports stating that the subcellular localization of caspase-7 both pro and active form is cytoplasmic however there are reports where it states that after activation caspase-7 translocates to the nucleus like caspase-3?

Mohamed-Mourad Lafifi

Dear Puneeta Bathla,

I suggest you to see links in subject.

-Different Subcellular Distribution of Caspase-3 and Caspase-7 ...

www.jbc.org/content/273/18/10815.full

-Human Caspase-7 Activity and Regulation by Its N-terminal Peptide

www.jbc.org/content/278/36/34042.long

-Calpain-1 Cleaves and Activates Caspase-7 - NCBI - NIH

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2757245/

-Compartments of Viable Mast Cells Active Caspase-3 Is Stored within ...

www.jimmunol.org/content/jimmunol/191/3/1445.full.pdf

-Non-apoptotic functions of caspase-7 during osteogenesis | Cell ...

https://www.nature.com › cell death & disease › original article

Best regards

Badges
Science topic

More Punita Bathla's questions See All

How to calculate the times a random experiment needs to be conducted (diff samples of same population) for generalisability?

any scientific method?

20 January 2024 7,632 0 View

Is it acceptable to add more conclusions in a conference paper that were not discussed due to shortage of space?

Hi, My research project had numerous results and because the conference paper is usually just 5-6 pages long, I could not discuss all my results in it. However, is it still possible to mention...

04 May 2020 721 5 View

Missing atoms from CONTCAR file after the simulation run for geometry optimization. Why??

I am trying to run geometry optimization of a catalyst structure and a reactant on its surface. AFter the simulation, I observed 10 atoms (2 Oxygen and 8 hydrogen atoms) from the reactant molecule...

23 April 2020 2,959 0 View

How long will it take to digest the food in the absence of digestive enzymes in our body?

We all know that digestive enzymes speed up the chemical reaction and helps in food digestion. I was wondering if in general it takes 6 h to digest a food with the help of digestive enzymes then...

04 October 2019 5,655 3 View

Can anyone suggest me a better coating reagent other than Poly-L-Lysine for the adhesion of HEK-293T cells?

I recently coated the 96 well plates with 0.1 % w/v (1:10) dilution of Poly-L-Lysine for seeding the HEK-293T cells. However, I found that the attachment of the cells to the surface is not...

24 May 2019 476 2 View

How to perform cell based assay in 96-well plate using HEK-293 cells as the cells shows poor adhesion and comes off quicking ?

I have to perform fluorescence based assays in 96-well plate using HEK-293 cells. The experimental procedure involves transfection in cells and then treatment with drugs for testing drug efficacy....

01 May 2019 3,738 9 View

Does all enzymes are synthesised by ribosomes in zymogen form or are there expections when enzymes are synthesized in active form?

Enzymes are synthesised as zymogens and then make a transition to active form when required, is this true for every enzyme or are there any exceptions to that?

17 March 2019 4,480 3 View

Why am I seeing expression all over the cell while expressing EGFP-N1 plasmid in MCF-7 cells?

I have expressed EGFP-N1 plasmid (1ug) in MCF-7 cells. Upon taking the confocal images under 40x oil I have seen the expression all over the cell? What is the possible reason for that? Can anyone...

27 February 2019 6,380 6 View

Is it possible to obtain a dimer in SDS-PAGE reducing gel?

I have purified a monomeric EGFP- His protein with a molecular weight of 30 kDa. But, instead of 30 kDa I am getting a thick band of 60 kDa in SDS-PAGE reducing gel. what is the possible reason...

30 January 2019 5,899 2 View

Why the Ni-NTA purified His-tagged EGFP protein is coming at 60 kDa instead of 30kDa on SDS-PAGE gel?

I have purified a EGFP-His tagged protein which was there in pET15b vector using Ni-NTA column. The concentration obtained was way too high around 5mg/ml. Before the running the SDS-PAGE the...

30 January 2019 7,380 2 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

Why I have this weird green signal in the nucleus?

Hi, In a few wells and cells, we’ve noticed an unusual signal in the nucleus using both eppifluroscence & confocal microscopes. Does anyone know why this is happening? It occurred twice in...

10 July 2024 1,870 0 View

What does it mean when NucBlue disappears completely during live cell imaging?

Using confocal imaging, I'm investigating the effect of a particular compound on mitochondrial membrane potential in live cells. I've stained the cells with varying concentrations of the compound...

29 June 2024 7,546 4 View

How good can CI users localize sound?

It is probably pretty variable between CI users, any ideas?

24 June 2024 6,879 1 View

Does anyone have experience with immunofluorescence of endoplasmatic reticulum?

Hi! I have performed several immunofluorescences of cultured ARPE-19 cells with different antibodies expecting in each one a cytoplasmic localization. However, it seems to localize in the...

06 June 2024 7,764 1 View

Is CFSE retained in cell nuclei?

Does anybody know whether CFSE is retained in cell nuclei after treatment of cells with pepsin solution in HCl?

29 May 2024 1,357 0 View

Universities that offer PhD programs specializing in website and game localization?

I'm seeking recommendations for universities that offer PhD programs specializing in website and game localization. Any suggestions or pointers to relevant departments or universities would be...

16 May 2024 4,880 1 View