Punita Bathla

13 Questions 12 Answers 0 Followers

Questions related from Punita Bathla

Why do I see an increase in the renilla luciferase (RLuc 8.6) activity in apoptosis cells as compared to heathly cells?

MCF-7 cells are transfected with caspase-3 Rluc 8.6 plasmid and the cells were treated with staurosporine drug in order to induce apoptosis. I see increased luciferase activity in apoptotic cells...

11 March 2020 6,396 2 View

Why am I seeing expression all over the cell while expressing EGFP-N1 plasmid in MCF-7 cells?

I have expressed EGFP-N1 plasmid (1ug) in MCF-7 cells. Upon taking the confocal images under 40x oil I have seen the expression all over the cell? What is the possible reason for that? Can anyone...

28 February 2019 6,421 6 View

Why does GFP dimerises at higher concentrations (5mg/ml) ? What is the nature of the interaction which causes this dimerization ?

I have purified EGFP-His tagged protein. The protein is purified in a dimer form. The concentration of the purified fractions are high (5mg/ml). I want to know what causes the dimerization and...

02 February 2019 5,296 0 View

What happens to lysosomes when cells are treated with staurosporine or any other apoptotic insults ?

I treated HEK-293 cells with 1 uM staurosporine for 3 hours. Cells were labelled with lysotracker. In the untreated cells labelling of lyso-tracker was seen however in staurosporine treated cells,...

26 November 2018 4,767 0 View

How many lysosomes are present in a typical cancer cell and what is the diameter of a single lysosome?

How many lysosomes are present in a normal cell versus cancer cells?

22 November 2018 8,684 3 View

Can anyone suggest me a source from where I can get a cell line stably expressing my enzyme of interest tagged to a reporter protein?

I want MCF-7 cells stably expressing caspase-3 GFP protein. Could anyone suggest me from where I can outsource this?

31 October 2018 7,289 4 View

What is the subcellular localization of procaspase-7 and active caspase-7?

There are reports stating that the subcellular localization of caspase-7 both pro and active form is cytoplasmic however there are reports where it states that after activation caspase-7...

29 June 2018 3,235 1 View

What is the optimum concentration of TNF-alpha protein required for inducing apoptosis in MCF-7 cells ?

Looking for the optimum concentration of TNF-alpha protein required for apoptosis induction.

07 June 2018 6,669 2 View

Which caspase enzyme in the caspase family is not known to be involved in apoptosis pathway?

Caspase family consists of several caspase enzymes from (1 to12). Caspase are known to be actively involved in the apoptosis pathway. I want to know the exception of if there is any caspase in the...

05 April 2018 3,130 1 View

Does all caspase enzymes in the caspase family are invovled in apoptosis pathway?

Caspases are known to play a major role in apoptosis pathway. Is there any caspase among the caspase family whose role in apoptosis pathway is not known yet?

26 February 2018 5,723 0 View

Apoptosis induced at which stage of the cell cycle?

Stage of cell cycle at which apoptosis can be induced

21 February 2018 9,156 4 View

Can I use Lipofectamine Stem reagent for transfecting MCF-7 Cell line?

Lipofectamine Stem reagent (Invitrogen) on the website it is mentioned that the reagent is for transfecting stem cells with low toxicity. So can I use this for transfecting MCF-7 cell line?

27 December 2017 3,514 2 View

What is the best reagent to induce necroptosis in HEK-293 cell line?

There are certain reagents which induces apoptosis in certain cell lines and necroptosis in others. It is also known that both these pathway utilizes the same death receptor. Here, I'm looking for...

26 December 2017 4,887 2 View