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Questions related from Punita Bathla
MCF-7 cells are transfected with caspase-3 Rluc 8.6 plasmid and the cells were treated with staurosporine drug in order to induce apoptosis. I see increased luciferase activity in apoptotic cells...
11 March 2020 6,391 2 View
I have expressed EGFP-N1 plasmid (1ug) in MCF-7 cells. Upon taking the confocal images under 40x oil I have seen the expression all over the cell? What is the possible reason for that? Can anyone...
28 February 2019 6,413 6 View
I have purified EGFP-His tagged protein. The protein is purified in a dimer form. The concentration of the purified fractions are high (5mg/ml). I want to know what causes the dimerization and...
02 February 2019 5,289 0 View
I treated HEK-293 cells with 1 uM staurosporine for 3 hours. Cells were labelled with lysotracker. In the untreated cells labelling of lyso-tracker was seen however in staurosporine treated cells,...
26 November 2018 4,761 0 View
I want MCF-7 cells stably expressing caspase-3 GFP protein. Could anyone suggest me from where I can outsource this?
31 October 2018 7,282 4 View
There are reports stating that the subcellular localization of caspase-7 both pro and active form is cytoplasmic however there are reports where it states that after activation caspase-7...
29 June 2018 3,229 1 View
Looking for the optimum concentration of TNF-alpha protein required for apoptosis induction.
07 June 2018 6,662 2 View
Caspase family consists of several caspase enzymes from (1 to12). Caspase are known to be actively involved in the apoptosis pathway. I want to know the exception of if there is any caspase in the...
05 April 2018 3,126 1 View
Caspases are known to play a major role in apoptosis pathway. Is there any caspase among the caspase family whose role in apoptosis pathway is not known yet?
26 February 2018 5,718 0 View
Stage of cell cycle at which apoptosis can be induced
21 February 2018 9,149 4 View
Lipofectamine Stem reagent (Invitrogen) on the website it is mentioned that the reagent is for transfecting stem cells with low toxicity. So can I use this for transfecting MCF-7 cell line?
27 December 2017 3,509 2 View
There are certain reagents which induces apoptosis in certain cell lines and necroptosis in others. It is also known that both these pathway utilizes the same death receptor. Here, I'm looking for...
26 December 2017 4,882 2 View