After making an extract from the plant, I added Bradford and this strange structure appeared. I used a centrifuge, but it did not disappear. Has anyone had a similar situation and knows what happened and why this structure appeare?
My negative control cells had some damage, they are neoplastic cells and my anaplastic cell had even more damage in the negative control. Ok, but my question is, is there much difference between...
27 May 2024 7,529 2 View
Hello guys, I have performed experiment on optimisation of PCR for one of the SNPs and while performing electrophoresis, the issues occurred that I'm trying to figure out right now, and need some...
24 March 2022 1,496 6 View
Dear All, Have You ever faced problems with atherosclerotic plaque staining? Yesterday I stained mice aortas full of plaques. Very few of them are not stained. So then I repeated the whole...
08 February 2022 8,618 3 View
I have a primary antibody with size: 50 μg and concentration 0,5mg/ml and I shoud have used to IHC - Paraffin (3.75 µg/ml). I understand that in this case I should use a ratio of 1:...
03 March 2021 3,947 3 View
Can anti rabbit antibodies be used in a study where the study model is a rabbit? Does that make any sense? Only one producer also reacted to the rabbit's reactivity...
23 October 2019 3,088 3 View
Can anybody recommend a good multi-parameter portable meter? I will measure dissolved oxygen, pH, ORP, conductivity, and temperature (in surface water and groundwater). I used to use WTW 350i, but...
03 April 2018 4,966 13 View
For my synthesis I need the Hodogaya carbon nanotubes, which unfortunately are not produced anymore, and therefore, impossible to buy. Does any of you have the Hodogaya MWCNTs in stock and is...
03 October 2016 9,882 2 View
why people are so prone to create false memories and then convince themselves/believe that it really happened?
27 April 2016 8,639 8 View
I may try with values 0.3-0.35 for my models in Abaqus but I prefer to utilize verified values.
29 February 2016 8,733 4 View
What are the Viewfactor and Clearance in Radiation, in Contact Property Options? I am trying to explore the possibility of modelling the heat transfer (in Abaqus) from the skin surface into textiles.
23 November 2014 399 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...
31 July 2024 8,666 3 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
how can I extract my bibliography from researchgate ?
28 July 2024 6,737 1 View