In our pilot study, we have to analyze human RBCs for their osmotic fragility. We have started to do the basic procedure, i.e. preparation of sodium chloride solutions with various concentrations and addition of a blood drop and inspection of the preparation after 1 hour for hemolysis by naked eye. If anyone knows the standard protocol to conduct the RBCs osmotic fragility test with the highest accuracy, please help us.
Kindly follow the link and osmotic fragility test protocol. I believe, it will be helpful for you.
Best regards,
Babu
https://www.ncbi.nlm.nih.gov/books/NBK190583/
Please find details in these publication. If you have any doubt you can contact me.
Sarkar, M.; Barari, S. K., Mondal; D.B., Nandankar, U. A.; Basu, A.; Mohanty, T. K. and Ray, S. (1999). Effect of anti-coagulants on osmotic fragility of erythrocytes of yak (Poephagus grunniens). The Veterinary Journal. 157(1): 91 -93.
Sarkar, M.; Mondal, D. B.; Mohanty, T. K. and Pal, R. N. (1999). Effects of temperature and pH on osmotic fragility of erythrocytes of yaks. Australian Veterinary Journal. 77 (3): 188 - 189.
If you want to do de Hereditary Sepherocytosis diagnosis the golden standard is the eosin-5’-maleimide (EMA) binding test. For the osmotic fragility you can use the Acidified Glycerol Lysis test or cryohemolysis test.
I advise you to consult the work of Narla Mohandas! I his the best expert in the rbc membrane field.
Classical publication on this subject is "Beutler E, Kuhl W, West C: The osmotic fragility of erythrocytes after prolonged liquid storage and after reinfusion. Blood 1982;59:1141–1147"
http://bloodjournal.hematologylibrary.org/content/59/6/1141.full.pdf
Kindly follow the link and osmotic fragility test protocol. I believe, it will be helpful for you.
Best regards,
Babu
https://www.ncbi.nlm.nih.gov/books/NBK190583/
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