Will be attempting to pass some parasites in vivo (suseptible mice) and was wondering what is considered acceptable in the field.
Zainab A Makawi
That is my question, has any of you guys seen a number that seems standard
Please have a look at:
Carrero JC et al., Protection against murine intestinal amoebiasis induced by oral immunization with the 29 kDa antigen of Entamoeba histolytica and cholera toxin. Exp Parasitol. 2010 Nov;126(3):359-65. doi:10.1016/j.exppara.2010.03.007. Epub 2010 Mar 19.
It is better to to give different doses according to weight of mices until prove the good induction of disese.
The cecal amebiasis was induced in some literatures by the injection of 3.0×105 troph/mL of E. histolytica parasite directly into the rat’s caecum.
https://www.pagepress.org/journals/index.php/vsd/article/view/5793/6353