What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

What is the state of the art with regards to machine learning in object tracking in video?

I've recently found some time to do some research and find this field particularly interesting. It's been a while since I looked at any research. Just wondering what the latest and greatest is.

29 January 2019 6,266 7 View

Applying to NSF grant, found similar idea previously funded by foreign org. Should I mention this to give credibility to problem, ignore, or give up?

I am planning on applying to an NSF (US) grant, but found that a similar idea to what I had in mind is already funded by a foreign institution. I am curious what the Research-Gate community would...

18 April 2017 8,341 2 View

Will a standard plasmid mini prep contain enough bacterial 16s rDNA to amplify in PCR with general 16s primers? If so, could this be avoided?

I am preparing to use some plasmid DNA to spike into soil samples at a known concentration as an internal standard to account for variable DNA extraction efficiency. I will also be looking at...

08 January 2015 5,213 3 View

Is it acceptable to separate mice in a drug trial on basis of baseline results, or should they be separated blindly?

I'm working with a colleague on series of experiments treating mice with compound A to see if their condition improves. Baseline behavior tests were administered to determine the difference...

08 May 2013 8,226 13 View

Is axisymmetric problem just the same as a normal planar problem?

Hi, I wanna study the diffusion problem in a spherical structure with FEM. I think it is natural to use the axisymmetric element. But I am not sure that whether the axisymmetric feature of the...

01 January 1970 7,830 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View