Devin Camenares

4 Questions 15 Answers 0 Followers

Questions related from Devin Camenares

How do I efficiently process a text file that is over a billion lines long?

I am attempting a bioinformatics project in which I a processing, line by line, a text file that is over a billion lines long (about 67 Gb). Each line contains a chromosome number, a nucleotide...

10 June 2023 3,751 3 View

What is the source of Golden Gate side products?

I am attempting a Golden Gate assembly with 4 pieces (including the backbone). I have sequenced each piece and verified the presence of BsaI sites on the end, with compatible overhangs. The...

29 July 2022 9,124 0 View

How much more sensitive is a Southern Blot versus Ethidium Bromide staining in 2D DNA gels?

I am exploring the use of 2D gel DNA electrophoresis for analyzing plasmid DNA replication in vitro. I have found a number of articles that utilize this technique for this purpose, but they all...

29 December 2021 4,203 2 View

Applying to NSF grant, found similar idea previously funded by foreign org. Should I mention this to give credibility to problem, ignore, or give up?

I am planning on applying to an NSF (US) grant, but found that a similar idea to what I had in mind is already funded by a foreign institution. I am curious what the Research-Gate community would...

19 April 2017 8,472 2 View