Hi there, I was wondering if anybody has had success in the production of 3D cardiac spheroids in scaffold-free culture (e.g hanging drop) using immortalised cardiomyocytes?
I'd ideally like to produce spheroids containing AC16 cardiomyocytes (an immortalised human CM cell line), along with HUVEC cells and primary cardiac fibroblasts.
I haven't been able to locate evidence supporting the use of immortalised cell lines such as the AC16s in the generation of spheroids - with most protocols instead using iPSC derived CMs.
If anybody has tried this or has any information that may help please let me know! Cheers
Hi
Check this.
Article 3D In Vitro Platform for Cell and Explant Culture in Liquid-...
Also, another paper showed culturing in 3D bioprinted material,
Article 3D Bioprinted Spheroidal Droplets for Engineering the Hetero...
you may think of sodium alginate to encapsulate or can try to use serum-free media with suitable growth factors to develop some method of culturing AC cells spheroids
Regards
Saurabh
Hi Devin,
I hope that you are well!
Sorry for the late reply, I came across this question whilst looking for information on AC16 spheroids.
Yes this technique of producing spheroids with AC16, endothelial cells and fibroblasts is possible. It is a model I have used in my PhD and can produce some nice data.
If you are still interested in this technique, I would be happy to further discuss.
thanks,
Rob
Robert Bentley thanks for the reply Robert! I've sent you a message to discuss further :)
Cheers,
Devin
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