What is the source of calcium and iron in food of milk giving animals?

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What is the unit of light pollution ?

How light can be polluted

09 October 2019 2,867 5 View

What are the problems tissue engineered dental enamel and dentin could not be created in desired shape and size ?

This could prove to be a good alternative treatment for dental caries

09 October 2019 8,947 2 View

Which are the second line treatment drugs shifted to first line of treatment of epilepsy in human?

What are the new drugs supposed to treat epilepsy

08 September 2019 2,951 5 View

How to identify the sources of enzymes in analytical chemistry of biological processes in human?

Presence and quantification of enzymes is possible but source determining is difficult as source may be microbial/ viral/ healthy or diseased tissue

07 August 2019 1,813 4 View

What are the effects of extreme fluorosis on health/ histological pictures of dental pulp in human teeth?

Fluorosis is affecting osseous tissue and may result in crippling situation of person with difficulties in walking

07 August 2019 9,676 3 View

How much is the atmospheric pressure in sky distant from the planets and satellite?

Has anyone ever tried to see this pressure

06 July 2019 3,339 3 View

In which spectrum of electromagnetic radiation dark energy belongs?

Dark energy may be towards transparency and difficult to see

06 July 2019 1,294 2 View

How much is the change in size of molecules in gaseous state under extreme pressure and vacuum?

Under pressure and vacuum molecular motions are known but size is unknown as far as I am having knowledge

06 July 2019 3,794 3 View

The temperature difference between malignant lesion of internal organ and surrounding healthy tissue and malignant and benign tumours in human ?

How to measure the temperature during surgery in sterile area and which instrument is suitable for this

03 April 2019 3,219 1 View

The images of sun in day time and noon are real or refracted?

In water wooden stick will show two components of image one without refraction/ real and one refracted What is the status of sun we are seeing

03 April 2019 5,356 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View