The Bradford reagent is strongly acidic (8.5% phosphoric acid or about 1 M). Unless your sample is in a very concentrated pH buffer, the pH won't make any difference because it will be overwhlemed by the acidity of the reagent.

Hello,

You may prepare 1mg/ml bovine serum albumin (BSA) stock in water or in the buffer in which your protein (sample) of interest is currently in.

Anoop

Hello Adam B Shapiro, some samples will be in HCL solution at ph 1 and others in NaOH at ph 11. Thank you for your contribution

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