How can I calculate excitation energy of a pi-conjugated organic compound in selected solvent using state specific PCM and CAM-B3LYP in Gaussian 09?

"We were trying to calculate excited state properties in solvent environment (e.g. ethanol) using CAM-B3LYP/6-311++G(d,p) and state specific PCM method in GAUSSIAN 09. There's however a challenge...

30 August 2020 138 2 View

Could anyone give me a clue on the identification of this moth?

It has been found feeding on Eucalyptus leaves in Indonesia. Family or genus, maybe? Thank you.

25 December 2019 6,971 3 View

Unusual qPCR Amplification Curves?

Hi all, May I have some suggested reasons why I am obtaining amplification curves (in pink) with unusual shapes (particularly near the baseline) and plateaus of various heights? This is a...

25 December 2019 9,297 3 View

Why do I achieve low intensities in oxygen evolution reaction?

Hello, I'm performing oxygen evolution reaction in a three-electrode system (glassy carbon working electrode, Ag/AgCl (3M) reference electrode, and Pt as counter electrode), with 0.1 M KOH...

01 September 2019 407 37 View

Does varying the excitation wavelength affect the emission wavelength?

I excited a fused aromatic conjugated naphthalene-based system from 240-360 nm and the emission bank was continuously red shifted from 500-800 nm. The absorption maximum is around 360 nm.

09 May 2017 6,270 4 View

Is a PCR efficiency of 95% to 105% essential for qPCR?

21 September 2016 1,351 5 View

Which is the amount of DNA (ng/ul) expected for 1mg of fresh tissue. For example one mosquito sample?

There is some work on the expected amount of DNA per milligram of tissue.

19 August 2016 4,865 7 View

There is DNA really in mosquitoes kept on mothballs or it's degraded? Someone has amplified DNA from old insects preserved on naphthalene?

I amplifly ITS2 DNA from old mosquito samples preserved on naphthalene using antioxidant, but the target sequence does not amplify, only nonspecific fragments, smaller and more stable. This DNA...

16 August 2016 9,181 3 View

I have a PCR purified fragment for sequencing but when I sequenced it, I suspect there are two amplicons. What cloning strategy can I use to fix this?

A fragment of DNA from a genomic prep was amplified by PCR using specific primers. This fragment was PCR purified and sent for sequencing. The sequencing results however indicated that possibly...

11 November 2015 6,149 13 View

What is the best method to extract DNA from preserved mosquitoes on naphthalene (dry) for a long time?

I have succeeded in extracting DNA from mosquitoes kept on silica or freezer, using appropriate kits. However, in the older samples and preserved on naphthalene, the extracted product is...

12 August 2015 8,555 8 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

What are effects of steel jacketing on flexural strength?

Dear all, If steel jacketing is used to retrofit a reinforced-concrete (RC) column, can we use it to improve flexural strength? I understand that we leave gaps between the ends of the jacket and...

22 February 2021 1,906 5 View

How to model steel jackets using SeismoStruct?

Hi all, Does SeismoStruct support modeling rectangular RC columns retrofitted with steel jackets (e.g. rectangular, elliptical) ? Any suggestions for other software (apart from...

20 February 2021 4,521 1 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View