Has anyone had solubility issues for reserpine in Meuller Hinton Broth/agar @ 10mg/L (higher conc). I prepared a working stock in DMSO. It seems to dissolve in DMSO but precipitates instantly in MH broth.
Any suggestions?
Dear Kshipra,
Reserpine has limited solubility in MH broth:
The emergence of ciprofloxacin resistance was strikingly suppressed in the presence of reserpine. Whereas in the absence of reserpine 135 colonies of S. pneumoniae ATCC 49619 were obtained in medium containing three times the MIC of ciprofloxacin and 19 colonies were obtained in four times the MIC of ciprofloxacin, in the presence of reserpine only 3 colonies resistant to three times the MIC (a 45-fold reduction) were obtained, and no colonies resistant to four times the MIC were obtained. This dramatic effect could not be attributed to a toxic effect of reserpine. The MIC of reserpine for strain ATCC 49619 is greater than 40 μg/ml, the solubility limit for this drug in cation-adjusted Mueller-Hinton broth. Additionally, reserpine affected neither the colony-forming ability nor the colony size of S. pneumoniae plated in the absence of ciprofloxacin.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC89246/
Hoping this will be helpful,
Rafik
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