Kshipra Chandrashekhar

4 Questions 5 Answers 0 Followers

Questions related from Kshipra Chandrashekhar

How to design blocking oligos for NGS hybridization capture assays targeting viral sequences?

When designing blocking oligos for NGS hybridization capture assays, would it be sufficient if the sequence of the oligos is complementary to the NGS platform specific adapter sequence? Just...

18 October 2023 2,145 0 View

What is the solubility f reserpine in Mueller Hinton Broth?

Has anyone had solubility issues for reserpine in Meuller Hinton Broth/agar @ 10mg/L (higher conc). I prepared a working stock in DMSO. It seems to dissolve in DMSO but precipitates instantly in...

08 January 2016 7,011 1 View

Can anyone exist with coexpression analysis of prokaryotic transcriptome data?

Hello, I was wondering if there is any open source software/module available to carry out coexpression analysis of bacterial RNA seq data. I have performed DE seq for differentially expressed...

05 August 2014 4,993 3 View

During enrichment of RNA using MICROBExpress, if I don't get rid of 16-23 srRNA can I use the same elute with another kit to re-enrich he sample?

Can the eluted (incompletely enriched) mRNA be used again as starting material for another kit like say, Ribo Zero, or do I need to use a different set of total RNA altogether?

04 April 2014 184 2 View