Hello,

1.Colorimetry

This method is based on spectrophotometry and is the most common low-cost method currently deployed.

Detection limit: variates

2. Voltammetry: Ion Selective Electrode

This method involves applying a potential to a system of electrodes and measuring the resulting current created from the interaction with select ions. These are handheld devices which can be used multiple times.

Detection limit: about 2 ppb

3.Biosensors (e.g. DNA-based methods, Enzyme reaction mechanisms)

This method leverages microorganisms, enzymes, microspheres, and nanomaterials that have been developed to detect heavy metal.

Detection limit: 2 ppb

4.Biosensors (modified bacteria)

Example from the company FredSense: utilizes a tunable bacteria that can provide ultra-sensitive measurements of trace chemicals in a water. Currently have arsenic, lead, iron, and manganese.

Hello,

1.Colorimetry

This method is based on spectrophotometry and is the most common low-cost method currently deployed.

Detection limit: variates

2. Voltammetry: Ion Selective Electrode

This method involves applying a potential to a system of electrodes and measuring the resulting current created from the interaction with select ions. These are handheld devices which can be used multiple times.

Detection limit: about 2 ppb

3.Biosensors (e.g. DNA-based methods, Enzyme reaction mechanisms)

This method leverages microorganisms, enzymes, microspheres, and nanomaterials that have been developed to detect heavy metal.

Detection limit: 2 ppb

4.Biosensors (modified bacteria)

Example from the company FredSense: utilizes a tunable bacteria that can provide ultra-sensitive measurements of trace chemicals in a water. Currently have arsenic, lead, iron, and manganese.

Hey Karen,

Thanks so much for your reply!

Could you please give me more information about the Colorimetric method in the determination of Serum conc. of Lead?

Also, do you recommend a certain published article that have used this method?

Cheers,

Radhwan

Hello, just use google search and you will see a lot of methods . Just see by link below. https://www.google.com/search?ei=wBo8XKy4O4TjkgWmjK3QBA&q=colorimetric+method+for+detection+of+lead+&oq=colorimetric+method+for+detection+of+lead+&gs_l=mobile-gws-wiz-serp.12..35i39j33i22i29i30.4212.44005..45307...4.0..0.257.1682.0j4j4......0....1.........33i160.XN-KArDD_f8

Example of method attached.

Good luck!

Colorimetry or AAS

Dear Dr Alzidan

Lead determination in serum is not useful. Full blood levels (PbB) are necessary as they are more representative than Pb in serum, and all reference values are for PbB.

Any validated, published method with flameless graphite furnace atomic absorption spectrophotometry is fine.

Unfortunately all other (older and cheaper) methods are not sufficiently reliable for clinical application. Maybe you could buy a good second hand FAAS somewhere?

I wish you much success.

Kind regards,

Professor Frederik A. de Wolff

Leiden University Medical Centre

The Netherlands

Dear Professor Frederik A. de Wolff,

I posted my question with hopes to get such a thorough answer as yours.

I am grateful for the valuable information you have shared with us.

Best wishes,

Radhwan

colorimetricd methods may not be wufficiently sensitive in many cases. iF want a true value deterrmine it in red cells, afer centrifugation..

Thank you Luis Bertllo

..