Hi,
I am working on developing type 2 diabetic rats (Sprague Dawley) using High-fat diet followed by a low dose of STZ injection I.P (30 mg/kg ).
so those who have previous experience with this model, I would like to know the dose of STZ that was used? and in case of not developing diabetes after the first injection, will you follow up with other injections in consecutive days or following specific intervals?
Dear Dina,
The ideal dose of Streptozotocin (STZ) for inducing type 2 diabetes in rats is widely debated and varies depending on the strain of the rats and the study design. However, the commonly used dose for Sprague Dawley rats is 50-60 mg/kg.
In your specific study design, using a low dose of STZ (30 mg/kg) followed by a high-fat diet, it is possible that you may not achieve the desired level of hyperglycemia. In such a case, you could consider increasing the dose or repeating the injection after a specific interval, such as every other day for 2-3 injections. However, it is essential to note that repeated injections of STZ can result in severe toxicity and should be cautiously approached.
It is recommended that you monitor the rats' blood glucose levels regularly to determine the effectiveness of the treatment. Suppose the rats are not developing diabetes after the first injection. In that case, it may also be worth considering other factors that could affect the development of diabetes, such as diet composition and feeding schedule.
Thus, the ideal dose of STZ for inducing type 2 diabetes in rats may vary depending on the study design and the strain of rats being used. It isessentialt to monitor thrats' rats' blood glucose levels regularly to determine the effectiveness of the treatment.
I hope this helps. Yours sincerely,
Edgar M Cambaza
Dina Zidan
Did you start your research using STZ? I need your recommendation as I am going to conduct a work wit STZ for T2DM
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