Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

RNA extraction from exocrine pancreas?

Hi everyone ! Does anyone have any experience in extracting RNA from exocrine pancreas (which is very high in RNAse)? The pancreas is frozen tissue. I wonder if RNAse is also high in the nucleus,...

31 January 2021 390 2 View

Cell culture Vitamin D free media ???

Dear researchers, I am working onn the influence of vitamin D on cancer. I was wondering whether ther is Vitamin D free media , since I wanna be completly sure of my negative control without...

01 November 2020 582 2 View

When you have a protein as a start, What are the best bioinformatics tools to identify candidate ligands?

There are many tools to identify the protein target for a ligand. When I start with a protein, what are the best tools to figure out the possible chemical ligand for this protein?

09 August 2020 9,048 3 View

Western blot , Blocking step milk vs BSA ?

concerning the westrn blot ..........I am diluting my antibodies in 5% bsa , but I get very high backgroud though I an diluting the secondry antibody in 5% milk . My question is . Can I block the...

13 April 2020 297 4 View

What is the simplest method to determine the serum concentration of Lead?

Is there a simple, inexpensive method that can be used to accurately measure the serum concentration of Lead--other than the atomic absorption spectrophotometry (AA: graphite furnace and flame)...

12 January 2019 3,187 9 View

What is the simplest method to determine the serum concentration of Lead?

Is there a simple, inexpensive method that can be used to accurately measure the serum concentration of Lead--other than the atomic absorption spectrophotometry (AA: graphite furnace and flame)...

31 December 2018 7,211 10 View

How do I know if my data obey Beer's law or not?

Hi all, I would like to know if the data, for a given drug, obtained from the UV/VIS Spectrometer obey Beer's law or not. Is there a general rule? Also, why an Absorbance Reading above one is...

29 December 2018 4,391 6 View

How can I calculate minimum effective conc. if I have dissociation constant ?

I have dissociation constant (Kd) for a drug- receptor complex. Can I calculate the minimum effective conc. (MEC) using this Kd? How also can I calculate the oral dose that will cause this MEC or Kd?

20 July 2018 695 3 View

ISI and Scopus indexed journals have been considered as having high quality peer-reviewed publications.?

ISI and Scopus indexed journals have been considered as having high quality peer-reviewed publications. Why do they get such good scientific reputation? Does it mean that non-ISI-indexed journals...

04 April 2018 2,060 11 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View