Have you ever dealt with contamination in a cell culture resembling cancer stem cell spheroids?

While maintaining cancer cell lines originated from cattle squamous cell carcinoma we are troubleshooting an unusual problem as we are getting some spheroid like structures in our flasks...

03 April 2014 174 3 View

What could be the alkaline phohosphatase activity of squamous cell carcinoma cancer cell line-derived spheroids?

Spheroids or spheres derived from cancer cell lines usually consist of proliferating & un differentiated cells. Assuming this, what can be the alkaline phosphatase activity of cancer derived stem...

02 March 2014 6,011 1 View

What is an appropriateness of studying secretome in early passage cancer cells derived from tumor tissue?

I am not sure whether my thinking is in the right way or not but I think early passages of cancer cells are more suitable tumor model of tumors in vivo because after some passages (5-15) cells are...

31 December 2013 8,652 0 View

For determining tissue similarity index in cancer cells, which earlier passage should we consider for NGS run?

When does the term transcriptome come in case of the cancer cells cultured in vitro? If we are thinking for similarity determination of the cancer cells in comparison to original tumor tissue from...

31 December 2013 9,353 2 View

What should be the splitting ratio after 1st subculture from primary culture of tumor tissues when aiming to establish a cell line?

Actually to establish a cell line from cancerous tissue primary culture, the splitting ratio sometimes depend on the no. of cells live in primary culture. To establish a cell line the no. of...

31 December 2013 3,181 1 View

Is it possible to get tumor tissue derived stem cells in a primary culture?

After primary culture from one tumor sample (of squamous cell carcinoma of cattle ) I found a lot of small round shining apparently nucleated (fine adjusted inverted microscope) cells partly in...

31 December 2013 4,384 6 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View