Sharadindu Shil

7 Questions 5 Answers 0 Followers

Questions related from Sharadindu Shil

Have you ever dealt with contamination in a cell culture resembling cancer stem cell spheroids?

While maintaining cancer cell lines originated from cattle squamous cell carcinoma we are troubleshooting an unusual problem as we are getting some spheroid like structures in our flasks...

04 April 2014 235 3 View

What could be the alkaline phohosphatase activity of squamous cell carcinoma cancer cell line-derived spheroids?

Spheroids or spheres derived from cancer cell lines usually consist of proliferating & un differentiated cells. Assuming this, what can be the alkaline phosphatase activity of cancer derived stem...

03 March 2014 6,087 1 View

Is it possible to get tumor tissue derived stem cells in a primary culture?

After primary culture from one tumor sample (of squamous cell carcinoma of cattle ) I found a lot of small round shining apparently nucleated (fine adjusted inverted microscope) cells partly in...

01 January 2014 4,421 6 View

What should be the splitting ratio after 1st subculture from primary culture of tumor tissues when aiming to establish a cell line?

Actually to establish a cell line from cancerous tissue primary culture, the splitting ratio sometimes depend on the no. of cells live in primary culture. To establish a cell line the no. of...

01 January 2014 3,223 1 View

What is the significance of secretome analysis of primary culture in biomarker discovery of squamous cell carcinoma of cattle?

Actually the primary culture (derived from tumor tissue biopsy) will consist of heterogeneous population of cells so will it be valid if secretome analysis is done?

01 January 2014 7,383 0 View

For determining tissue similarity index in cancer cells, which earlier passage should we consider for NGS run?

When does the term transcriptome come in case of the cancer cells cultured in vitro? If we are thinking for similarity determination of the cancer cells in comparison to original tumor tissue from...

01 January 2014 9,392 2 View

What is an appropriateness of studying secretome in early passage cancer cells derived from tumor tissue?

I am not sure whether my thinking is in the right way or not but I think early passages of cancer cells are more suitable tumor model of tumors in vivo because after some passages (5-15) cells are...

01 January 2014 8,685 0 View