Issues with my microglia primary culture?

Hi everyone, I'm isolating microglia from P1-2 mice and seeding microglia cells from 3 pups brains/T-25 flask. After seeding, I let this astrocytes-microglia mix culture grow for approx 2-3 weeks,...

06 October 2020 3,916 3 View

Do you have any suggestion for a easy and cheap experiment to quantify Cytokines?

I need to test the stimulation production of TNF alpha in the RAW cell line by LPS. I am looking for an easy and cheap experiment to quantify the serial dilution stimulation in this macrophage...

27 September 2020 5,366 6 View

Good antibodies for D1 and D2 striatal medium spiny neurons?

Hello! I am looking for good antibodies that help in clearly distinguish between D1- and D2-type medium spiny neurons (MSNs) in the striatum, by immunohistochemistry and immunofluorescent...

31 May 2020 5,009 3 View

Good antibodies for rat M1 and M2 microglia?

Hello! Do you know some good antibodies, suitable for IFL, for differently staining the M2 and M2 microglia phenotypes in rat tissue? I am searching for antibodies specific for RAT because all the...

27 October 2019 7,260 3 View

Anyone can help me about grayanotoxin analytical standards for LC-MS/MS?

I need to buy analytical standards for grayanotoxin I, II and III. I only find the type I. Roberta Merlanti

15 May 2019 2,628 4 View

Could someone help me to choose the right concentration and time of neutralizing IL1beta antibody after LPS treatment to block its signalling?

Thanks!

06 October 2016 4,648 3 View

Is THPS toxic towards methanogenic bacteria? In case it is, is there a "selective (more or less!) biocide against sulphate reducing bacteria?

Tetrakis Hydroxymethyl Phosphonium Sulphate and methanogenic bacteria

21 September 2014 8,375 2 View

Any alternatives to 3MA solubilization?

I have to use 3-MA in my cell culture at high concentration. II know that 3-MA is not very soluble in water, so the solution would be solubilize 3-MA in DMSO, but I know that DMSO generally...

08 June 2014 1,658 3 View

What types of transfection reagents do people use to transfect neurons, IPS derived neurons, astrocytes and neural stem cells?

Do more people use cDNA, siRNA, mRNA or miRNA? Would love to hear what people are using?

29 January 2014 1,147 7 View

What types of transfection reagents are you using to transfect neurons, astrocytes, IPS neurons?

cDNA, mRNA, siRNA, miRNA

27 January 2014 4,591 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Culturing Peziza sp?

Hi everyone, Any successful trials for getting some cultures from Peziza Fruitbodies? We have done several trials using usual medium of PDA/ MA, but all failed. Thanks in advance

29 December 2020 6,966 6 View

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home?

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home (freeware)? Chromatograms were made by Waters UPLC I-Class PDA-MS. At the workplace,...

14 May 2020 5,383 3 View

Waters ACQUITY UPLC - Issues with PDA?

I'm attempting to use a Waters Acquity UPLC prior to mass spec and I'm seeing really weird chromatograms. When I inject water, the PDA detector shows a steadily increasing signal (looks like a...

01 April 2020 4,117 2 View

How can I determine a bacterial colony forming unit?

I got samples from chicken gizzards fed with different oyster mushroom, and streak inoculated them on PDA plate after 10-6 serial dilution, now how can I determine the bacterial colony forming unit?

02 March 2020 4,823 3 View

Is there any specific method for fungal colony growing for DNA extraction ?

I want to extract the fungus isolate DNA for Sequencing and Identification as i have different isolates from fruit peel, so i want to know that is there some requirements for colony grow...

12 January 2020 8,128 3 View

I am finding trouble in isolation of Phytophthora infestans from potato leaves in PDA? What might be the reason? Which could be the best media?

I am finding trouble in isolation of Phytophthora infestans from potato leaves in PDA? What might be the reason? Which could be the best media?

31 December 2019 2,563 9 View

Can you help identify these fungal colonies on PDA plates?

I am a Commercial Cannabis Production student looking to identify these fungal colonies based on their morphology on PDA plates. These samples were taken from various locations on campus, and we...

10 November 2019 1,517 13 View

How can we use ECDA(Eicosadienoic acid) to detect amines?

Hello! My coworker have been working with spoilage tests and Ive been helping him. But these days we have a problem about ECDA solution. We couldnt get any colour change when we test it. So I hope...

06 November 2019 9,543 2 View

I need to culture Fusarium lactis on PDA agar. Does anybody got a protocol for PDA agar to culture fungus?

I am a plant science student working on Fusarium lactis which affcets peppers in glasshouses?

09 October 2019 2,379 3 View