Why Cutting force coefficients or the edge coefficients will be negative in few cases of machining operation?

In mechanistic modeling of cutting forces in turning, identification of cutting force coefficients and edge coefficients through experiments are very much essential. these coefficients can be...

05 October 2019 3,864 3 View

What can control gene expression after irradiation (besides DNA methylation)?

I'm studying a gene whose expression is regulated by DNA methylation. Cancer cell lines that express this gene show strong hypomethylation, while cells lines that don't express it show...

19 March 2019 7,845 2 View

How to solve scattering problem in sidewall of any pattern devices by using maxwell formulation without simulations through FDTD and COMSOL?

Actually i am looking some theoretical model who can solve the semiconductor scattering issues.

22 August 2018 3,062 2 View

Is it possible to get yield more than 100% in a chemical reaction....?

Is it possible to get yield of a chemical product more than 100%...? If so, how one can defend this situation? I am working on Condensation reactions using organic solvent free synthesis. For some...

22 June 2018 2,453 21 View

Can I transfect the only 147nt ssOdna 5'-3' (only sense strand)as repair template for Knockin experiment?

27 August 2017 3,856 2 View

Hi - does anyone have any advice about retrieving PBMCs from their suspension in RNAlater?

The PBMCs were suspended in RNALter and then stored at -80oc. Now I want to retrieve the PBMCs and then extract the RNA (inc the microRNAs) - does anyone have advice about centrifuge speeds/...

19 June 2017 6,038 3 View

Molecular weight changes with GPI Anchors

I'm studying a GPI-anchored protein and all of the antibodies against this protein are ~5-7kD higher than the expected molecular weight (expected is ~18kD and most antibodies give ~25kD). I'm...

21 May 2017 1,323 5 View

Does anyone help me out to clone the Oligo sequence in Lenti CRISPRv2 backbone plasmid ?

18 May 2017 6,919 1 View

In ABAQUS: How to apply pressure loads at different locations?

In ABAQUS: How to apply pressure loads at different locations?

07 May 2017 1,676 4 View

Is there any way to in vitro methods for checking to anticancer properties of plant extracted substance without using animal cell line or MTT assay?

I am working on the phara quantification of plant substance, facing some experimental aspect , alike no availability of animal cell culture setup. So, anticancer assay aspect completely non in...

20 January 2017 8,296 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View