What basic protocol can you recommend for making liposomes with a highly water insoluble drug?

I am new to liposome making. My drug (carbamazepine, mol wt. 236) is highly insoluble in the water. I tried to load it in bilayer using a basic protocol. After lipids(DMPC, DMPG, Cholesterol)...

23 November 2020 4,886 3 View

Does anyone has experience with Dextran-FITC uptake by cancer cells?

Please share the protocol for antigen uptake assay by cancer cells. I want to use Dextran-FITC to track the endocytosis by cancer cells. If anyone has the protocol or any suggestions kindly share.

25 October 2020 469 3 View

The right statistical test for microbiological data from food production environment?

I am analyzing the safety aspects of two food production sites producing similar products. I wish to compare these geographically distinct sites across seasons as well as processing steps. I have...

15 July 2019 550 3 View

Can anyone please suggest me code of LEACH algorithm in python.?

Can anyone please suggest me code of LEACH algorithm in python.?

02 July 2019 5,023 1 View

Nuclear fractionation protocol for ChIP-sequencing and Co-IP mass spectrometry?

The protein I am working on is a transcription factor and is abundant in nucleus. I want to do subcellular fractionation of the adherent tumor cells followed by ChIP sequencing in order to...

02 December 2018 5,978 1 View

Please suggest me the method to determine the thickness of coating on whole fruits and vegetables?

How can be the thickness of coating on fresh whole fruits and vegetables be determined?

04 November 2018 4,296 4 View

How do I understand what's oxidizing and reducing from cyclic voltammetry of my polymeric system?

Hello everyone, I am conducting cyclic voltammetry experiments on my polymeric composite system Polyimide/polysiloxane/graphene using a three electrode setup and 1M KOH electrolyte. I am obtaining...

26 September 2018 2,947 3 View

Trouble in amplifying a particular gene?

I have trouble in amplifying a gene. I am continuously trying to find a possible solution. All methods have failed me somehow. I was amplifying gene A using gene specific primers. This gene has a...

21 May 2018 4,961 8 View

Can anybody explain or compare the difference between callus and tumor?

Generally callus is undifferentiated mass of cells, Whereas tumor is uncontrolled growth of cells, some say both are comparable, but I disagree, any idea Note: I am talking from a plant biologist...

21 May 2018 8,456 3 View

How to make sure if your RNA has been converted to cDNA?

I am trying to amplify a gene from cDNA. The gene contains an intron, therefore, I am amplifying it from cDNA. When I amplified it using genomic DNA, it shows the desired band. But when I am...

04 April 2018 3,238 8 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View