Can anyone name this graph type?

I've attached a recreation of this graph type that I need to make for study reports. The clients have sent them to us in the past, but I am trying to make the reporting process more streamlined,...

15 February 2021 9,795 3 View

Can you correct the wt%/at% of an incorrectly assigned peak in EDS?

For instance, I get a spectrum and the software incorrectly assigns a Si peak as W. If I don't catch it on the software and only have the report (which lists both wt% and at%), is there a way to...

10 February 2021 7,665 4 View

Can my lysis buffer interfere with the quantification of DNA and RNA using Qubit2.0?

I am doing RNA and DNA extraction using the basic phenol / chloroform / isoamyl method to quantify RNA and DNA in aqueous phase, but first, I do a lysis with buffer (Tris-HCL 1M pH8; EDTA 0.5M pH8...

17 January 2021 1,453 3 View

Is it possible to entrain a particle(s) into a gap/crack/cavity within Ansys Fluent?

I am trying to simulate the entrainment of a discrete phase particle in Ansys Fluent. Essentially, the particles would enter a pipe containing water, and flow into a hole in the pipe, and become...

13 January 2021 4,789 3 View

Is it possible to model a pressure-activated sealant in Ansys fluent?

I am trying to figure out how you'd model a pressure-activated sealant within Ansys fluent. This would be able to seal over a small crack, represented by a pressure differential, on the inside of...

12 January 2021 2,504 3 View

Would a non-Newtonian fluid be the best way to approximate the flow of large particles in Ansys which can plug a leak?

I am trying to model the sealing of a leaking water pipe within Ansys fluent, using the insertion of a stream of particles into the pipe, which would be forced into the gap by the pressure...

12 January 2021 9,509 0 View

Salivary Cortisol levels validity & reliability?

Hey Ya'll, I am wanting to test salivary cortisol levels in my participants. its a lab value and I do not know if we need to answer validity and reliability questions? I could not find articles...

02 March 2020 4,983 3 View

DATA MANAGEMENT-where will data be stored?

Hello everyone, How would I answer this question? is it asking for a specific program or device? "I will be storing the data on my password locked laptop"?

02 March 2020 932 2 View

Insect rearing artificial diets ?

I am interested in the area of artificial insect diets for rearing. Particularly diets that can be manufactured and commercialised. Does anyone have expertise in this area or can comment on the...

05 January 2020 1,725 5 View

How do I boost the signal of an Alexa594-conjugated dextran?

I am working on a project tracing projections using an Alexa594-conjugated dextran. I am interested in very fine processes which I'm having difficulty distinguishing from my autofluorescent...

16 December 2019 8,003 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View