This question is based from the article entitled “ Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya” written by Kavuvalu, B.M.
I've attached a recreation of this graph type that I need to make for study reports. The clients have sent them to us in the past, but I am trying to make the reporting process more streamlined,...
15 February 2021 9,795 3 View
For instance, I get a spectrum and the software incorrectly assigns a Si peak as W. If I don't catch it on the software and only have the report (which lists both wt% and at%), is there a way to...
10 February 2021 7,665 4 View
I am doing RNA and DNA extraction using the basic phenol / chloroform / isoamyl method to quantify RNA and DNA in aqueous phase, but first, I do a lysis with buffer (Tris-HCL 1M pH8; EDTA 0.5M pH8...
17 January 2021 1,453 3 View
Hey Ya'll, I am wanting to test salivary cortisol levels in my participants. its a lab value and I do not know if we need to answer validity and reliability questions? I could not find articles...
02 March 2020 4,983 3 View
Hello everyone, How would I answer this question? is it asking for a specific program or device? "I will be storing the data on my password locked laptop"?
02 March 2020 932 2 View
I am interested in the area of artificial insect diets for rearing. Particularly diets that can be manufactured and commercialised. Does anyone have expertise in this area or can comment on the...
05 January 2020 1,725 5 View
I am working on a project tracing projections using an Alexa594-conjugated dextran. I am interested in very fine processes which I'm having difficulty distinguishing from my autofluorescent...
16 December 2019 8,003 3 View
After bwa assembly to the reference genome I end up with sorted and indexed BAM files, but I am interested in understanding how to properly obtain all exon consensus sequences (to produce...
08 October 2019 9,970 2 View
I am designing an experiment where I would like to cross mice expressing cre in my cell subset of interest with mice that express either cre-inducible diphtheria toxin receptor (DTR) or...
15 September 2019 7,237 1 View
Say for exam I have a fracture plate on a bone, and I want to find out the Max and Min and average von Mises stress at different locations of the plate and bone (e.g. different screw holes).
19 May 2019 162 3 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...
24 February 2021 5,430 3 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?
23 February 2021 3,840 3 View
Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...
16 February 2021 1,337 2 View
Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...
10 February 2021 1,797 5 View