I have done gel electrophoresis of my PCR product the results were shown like this there were no proper bands observed in the gel and some of the amplified DNA bands were stuck at the well region can anyone tell me what could be the reason for this?
What is your DNA template? It is from genomic or plasmid? I am asking, because the top band, just below wells looks like accumulation of your template. And you have a lot of it, in my opinion too much. The same can be with primers, you can see them below the last band of the marker. It could be one of the reasons.
Usually I prefer to run PCR with as low amount of template as I can. So probably good point would be to lower the template concentration, I dont know, maybe 10 to 50 times?
Hi,
the signal in your comb region could be the template. What did you try to amplify (size and template material) and how did it run like mix, amount of template etc?
what i think you should dilute your primers and template concentration.
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