Salman Hassan

3 Questions 3 Answers 0 Followers

Questions related from Salman Hassan

I purified my PCR products gel pic attached, I got my sanger sequencing results, the chromatogram is attached below why does it happen like that?

I purified my three PCR products, their concentrations were between 40-60 ng/ųL. Is there any reason why the sanger sequencing of my samples if failed? In the Gel From lift to right first two...

22 November 2023 1,419 3 View

Why is the Nanodrop concentration of Purified PCR Product is low while there are clear and visible bands of PCR unpurified products on 2 % agroseGel?

I amplified an exome on PCR for sanger Sequencing, and run it on 2 % Agrose gel without ladder, but size of the fragment is 545 bp. The bands were clear. After purifying it with SolarBio...

14 October 2023 9,771 3 View

How can we calculate the sample size for rare inherited genetical disorders?

i am writting synopsis at the moment. I am going to perform mutational screening analysis for inherited neurodevelopmental disorders. So, How can we calculate our sample size using openEPI...

14 June 2022 9,236 1 View