We are using QIAamp DNA mini kit(250) to extract cfDNA from whole blood. While checking DNA concentration in Qubit, continuously getting ' low concentration ' (TOO LOW) for multiple samples.
What's the reason behind it? Can anyone suggest alternate method or remedy?
Thanks in advance
Greeshma
Hi,
the amount of cfDNA can be different between individuals and may depend on the health status. Moreover, cfDNA is highly fragmented and this may cause problems if you use normal DNA isolation protocols/kits. I would suggest to use a specific kit for circulating nucleic acids or an optimised protocol. We have obtained good results using the NaI method.
Best,
Norman
I would agree this kit does not really seem to be for the proper size selection of cfDNA. Generally you should expect that at the broadest it will be 100-250bp and this kit appears to be for larger DNA fragments. If you want to use a kit I would suggest finding one that selects for those sizes and will exclude genomic DNA that is larger.
Respected Greeshma Thulasi , try using QIAamp Blood Mini Kit.
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