Greeshma Thulasi

5 Questions 12 Answers 0 Followers

Questions related from Greeshma Thulasi

How to extract each exon into separate file from a multifasta file?

I have a multifasta file containing gene sequence of HLA A gene. I want to extract each exon into separate fasta file. Example of data file is attached: >HLA:HLA00001 A*01:01:01:01 1098...

27 April 2023 7,783 3 View

What is the reason for getting low cfDNA concentration in blood serum, while using Qiagen kit?

We are using QIAamp DNA mini kit(250) to extract cfDNA from whole blood. While checking DNA concentration in Qubit, continuously getting ' low concentration ' (TOO LOW) for multiple...

28 October 2020 7,008 3 View

Could you please explain the workflow of NIPTmer in detail?

I would like to know the working of NIPTmer. what does "A.2. Creating blacklist of known polymorphisms" do? which are the vcf files to be supplied? I've a set of BAM files. I'm able to do the...

18 January 2019 534 1 View

Is there any specificity among p-values, FDR and fold change while selecting the cut-off values for Microarray differential expression analysis

While finding the differently expressed genes from the microarray data, which are the necessary parameters that we have to taken into account for a more satisfying result? Which are the...

11 April 2017 2,804 5 View

How to open SOFT file for GEO database?

I would like to know how to open the .soft file downloaded from NCBI GEO.

25 January 2017 5,387 2 View