I am following the method of Ruch et al, 1989. But I am getting increasing order of OD with the increase of standard (quercetin) concentration. Can anyone tell me the protocol of hydrogen peroxide scavenging assay ?
I am studying the spectrophotometric assay of glutathione peroxidase. I want to know whether I can prepared the reduced glutathione previously and kept at 4oC for further use or not.
04 May 2018 9,871 8 View
I want to check the antioxidant activity of ZnO nano particles and hydrogen peroxide scavenging activity is one of them. Can anyone give the protocol to check such activity of ZnO nano particles?
03 April 2018 3,729 3 View
I am synthesizing ZnO nanoparticles by using plant extract. I want to study the effect of pH on the synthesis and need to adjust the pH of the reaction to obtain the optimal pH for nanoparticle...
02 March 2018 1,669 7 View
I want to change the pH of zinc acetate solution. But when I add NaOH, a white precipitate was formed.
02 March 2018 1,903 1 View
I want to study the effect of radiation on malignant tumor cell and also evaluate the stress during treatment in mice model
10 November 2017 495 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...
01 August 2024 2,614 1 View
Hello Everyone! I am trying to create a metal hydride system where I am trying to create a temperature control with the emphasis on measuring the concentration of the hydrogen with the hydrogen...
29 July 2024 7,577 2 View
For people today, the enigma of the size of the proton still remains enigmatic. However, being myself a researcher in new physical theory which makes it possible to extend the different standard...
28 July 2024 7,823 1 View
In general, when performing a Tafel analysis for corrosion resistance measurements, a line voltage scan is performed from say -250 to +250 mV vs. OCV at low scan speed around 0.125 mV/s. However,...
25 July 2024 7,982 3 View
I have tried to saturate ferritic steel with hydrogen using the electrolytic method and H2SO4. However, the effect was very poor even after 3 days of "saturation". Can anybody help in choosing a...
24 July 2024 4,393 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View