Yes sufficient concentration. I use to count the viable cell chamber Neubauer Impruved,. 0.5 ml of cell suspension plus 1.5 ml of 0.5% tripan blue. After 10-15 min you can see dead and viability cells - dead cells are stained in blue.
I wish you success.
Boris
Hello
Yes that's right , 0.5 ml sufficient concentration.
Good Luck
https://pdfs.semanticscholar.org/fd06/e4de0fe47482b94c349e7c01fe45dff43f8d.pdf
https://www.researchgate.net/post/What_is_good_protocol_in_trypan_blue_assay#599c9ccecbd5c2189a7a8b12
0.5 % is enough.
We take 10 µl of 0.4 % of the trypan blue solution and 10 µ l of cell suspension ( 1 × 10^6 cells/ml) and mix in a vial. Keep it for 3-5 minutes. In the end, count for viable cells in hemocytometer in 10 µl of the above.
you can use your 0.5% solution. Anyway, best pipette at least 50 ul of your cell suspension and 50 ul of trypan in order to achieve proper mixing avoiding foaming. After mixing you should wait 5 minutes and count. You can use both hemocytometer or count cells by means of Fiji Cell counter.
see
https://cellculture.altervista.org/trypan-blue-dye-exclusion-viability-test/
to have more infos.
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