6 Questions 17 Answers 0 Followers
Questions related from Maria Lisa Garavaglia
I'm planning to purchase Human Coronary artery Endothelial Cell from Lonza or ATCC. Is it possible to re-freeze batches of cells at the first passages in order to mantein the cell colture for a...
21 March 2017 7,244 1 View
Do someone know if 16HBE cells can be cultured at the air-liquid interface (ALI cell colture) showing differentiation to a polarized epithelia? Are there protocols available on the www? tnks a lot
18 February 2016 139 2 View
What potential and/or current corrections should be applied to perforated patch recording? What about liquid junction potential?
10 June 2014 3,398 3 View
I've seen a few works, but do you think it is possible to observe the activation of ClCa in whole cell configuration after ATP (that should increase intracell calcium), even if you're buffering...
02 April 2014 7,321 1 View
I've to perform patch clamp exps on CaCC after stimulation of purinergic receptors. I've seen both APT or UTP is used, which one is preferred? I know that ATP solutions are quite instable and ATP...
31 March 2014 5,686 15 View
I need to perform patch clamp exps with fast perfusion system to study ligand gated channels in the whole cell configuration. Could someone suggest a good fast perfusion system? I was thinking to...
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