Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to properly compare two simulation methods?

I have two methods for doing Monte Carlo simulations. With both of them I have run serveral simulations and got the mean and variance of their results. I would like to determine whether both...

20 November 2020 5,732 5 View

Can you investigate psychometric properties of a questionnaire when the factor analysis is not-converging?

Dear All, First of all I hope everybody is doing good in these weird times. Second, I also have a question regarding a non converging factormodel. I translated a questionnaire from English to...

18 November 2020 9,367 4 View

How can I calculate percent concordant in Stata 14?

I did a logistic regression and now I need to know how to compute concordant pairs of the predicted probability of my Y variable. I want to do this because I need to know model fit. I did the...

20 October 2020 7,819 1 View

How to simulate in EnergyPlus after modifying the source code?

I've changed some equations in one of the .cc files of the source code in EnergyPlus (downloaded from GitHub), but I do know know how to implement that file in the EnergyPlus installation folder...

13 September 2020 4,539 1 View

Feline immunodeficiency virus (FIV) radioimmunoprecipitation assay (RIPA) protocol?

Can someone share an FIV RIPA protocol? I have FIV infected/control cat sera that I want to check for antibodies to FIV proteins. Looking for a cell transfection with...

31 August 2020 8,487 1 View

How to use Factor Scores to create an index?

Hello all, I have used Principal Component Analysis to create a new variable that is like an index of a personal characteristic. The factor loadings of the variables used to create this index...

11 August 2020 5,182 6 View

Should there be sex education in schools and institutes about female menstruation?

02 July 2020 5,724 13 View

What percentage would have decreased murders for gender violence in Spain in ten years?

Some of the factors for this to be possible would be: Complete secondary education for girls (and boys); delay the age of marriages to 18 years; women's economic autonomy and access to skills...

11 March 2020 3,837 3 View

Could you explain about Unilateral Renal Agenesis?

I have been diagnosed with URA after having a CT scan done. I have no health issues related to my sole kidney (the right one), I eat a healthy diet, drink more water and watch my intake of...

25 November 2019 7,347 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View