What is the protocol for stain neutral lipids, using Nile Red?

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Daniela Rodler

Dear Carmen,

you may find some useful information on the following website:

http://stainsfile.info/StainsFile/jindex.html

Regards, Daniela

Sergio Rossi

This also may help.

Ramsy Agha

Hola Carmen,

Checking this older reference might help you as well

Greenspan P, Mayer EP, Fowler SD (1985) Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol 100:965–973

http://jcb.rupress.org/content/100/3/965