I have transfected the skpc1 cell line, unfortunately in the lab here noone has done a puromycin concentration curve yet to assess 100 % killing of untransfected cells. Since i have not enough cell plates to do a concentration curve by myself until the begin of the selection i am wondering if someone of you has already done it and could help out. Thanks in advance!
The recommended concentration of puromycin for mammalian cells is generally 1-10μg/ml, but the optimal working concentration needs to be determined by a dose-response curve.
Hello Daniel
Generally, for adherent mammalian cell line you can use a concentration ranging from 2 to 5 μg/ml and for suspension mammalian cell line you can use a range from 0.5 to 2 μg/ml. As mentioned by Linna Green for your cell line you will have to set up a kill curve using various concentrations of puromycin.
Best Wishes.
- Badges
- Science method