I Can we use a modified version of standards items ?

Dear Researcher, I have used standards items from Burger(2009). Since in it all items where not their, therefore, I added few items based on literature reviews and got alpha value 0.9 which...

13 February 2020 7,661 5 View

How to determine sample size for questionnaire development ?

questionnaire contains 25 items in a single group.

28 March 2019 309 5 View

The best culture condition for Kit-225

27 June 2017 1,898 6 View

How can I overcome such problematic melt curve in qPCR?

01 January 2016 8,023 4 View

How to achieve better amplification in qPCR?

30 October 2015 4,000 8 View

If the data collected is classified into more number of classes, will it improve the accuracy of goodness of fit test? Why?

This question is with regards to the accuracy of goodness of fit test.

04 January 2015 7,404 3 View

For a standard second order transfer function, what is the equivalent time domain significance of Zeta >0.707?

Zeta>0.707 implies no resonant peak in frequency response. But I am unable to correlate what is the exact time domain effect of this? If Zeta= 0.707 acts like an 'important damping value' in...

09 December 2014 683 11 View

Can formalin mix with Triton-X in PBS to make 3.7% formalin with 0.1% Triton-X solution for intracellular staining?

A standard protocol for intracellular staining usually separate the fixation and permeabilization part. Can I do the fixation and permeabilization at one time by incubating cells with 3.7%...

16 July 2014 8,541 5 View

What is the difference between non-fat milk and BSA for primary antibody in western blot?

Some protocols mention primary antibodies should be diluted in 5%BSA whereas some protocols mention primary antibodies should be diluted in 5% non-fat milk. What is the difference? What is the...

25 May 2014 8,114 19 View

Can anyone help me with these unusual results when multicolor staining in flow cytometry?

Currently, I am doing a research on NKG2D ligand expression on cancer cells. I would like to use dual color staining, which are APC-conjugated Ab for ULBP-1 and PE-conjugated Ab for ulbp-2....

03 March 2014 5,885 19 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View