Hi everybody! I can't amplify housekeeping genes (GAPDH and GUS) from a RNA sample of the H596 cell line. The sample was quantified by Quibit and its quality was tested by gel electrophoresis. I have tried to use more DNA or dilutions of teh sample but I couldn´t obtain any results. I used MDA-MB-231 derived RNA as positive control and I obtained satisfactory results with this sample. I use the QuaniFast Probe RT-PCR kit (Qiagen). Can anybody suggest me what is the cause of my problem please? Many thanks