Does reducing the brain neuronal network as an inevitable aging event serve to consolidate memory tracking?

07 September 2020 6,188 2 View

How to calculate Leaf Water Conten Index (LWCI)?

I want to calculate Leaf Water Conten Index (LWCI) using Landsat or Sentinel data. I found several usefull formulas that use NIR and SWIR bands. But to estimate LWCI I have to have NIR and SWIR...

16 March 2020 9,322 4 View

Real Time PCR Analysis and Normalization?

If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...

18 December 2018 6,267 2 View

Gene Expression Analysis using R?

Hello fellow researchers, Can anyone recommend a package for R for gene expression analysis using R?

13 December 2018 5,140 5 View

Monoclonal antibody purity?

If monoclonal antibody would be obtained from ascites fluid during hybridoma expression in vivo -> protein A/G-sepharose purification, how much non-specific AB contamination and non-target...

04 December 2018 6,582 3 View

Does the plasmonic near field spread on the distance sufficient for the resonance light absortion (scattering)?

Cross section of light absorption and light scattering for noble metal (silver, gold) nanoparticle is higher than its geometrical cross section in case of plasmon resonance. It can be...

30 October 2018 5,235 3 View

Degenerate Primers to ITS Region (16S-23S rRNA) for NGS?

F: 50 -CTKTTGGGYYCCCKGRYYG-30 Rev: 50-CGCGTCCACTMTCCAGTTCTC-30 These are primers for NGS sequencing of bifidobacteria from a publication. If you substitute, for example K, with G or T then...

09 April 2018 6,351 3 View

How to obtain a precipitated powder of Poly-Amic Acid based on aromatic diamine and aromatic dianhydride?

How to obtain a precipitated powder of Poly-Amic Acid (PAA) based on aromatic diamine and aromatic dianhydride? It is well known that for several Aromatic Polyimides (PIs) in the course of...

22 December 2017 9,399 3 View

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film ?

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film

17 August 2017 1,331 1 View

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know?

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know? I know about stegoDB but can't find download link. Thanks

10 April 2017 8,150 9 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

Books on Monte Carlo methods?

Hi everyone. I took a basic course on Markov Chains, and know a little about Monte Carlo Stimulations and Methods. But I never got to the part of spreadsheets. If anyone can help direct me to a...

13 February 2021 5,261 3 View

Are there any compounds that can increase the viability and stimulate cell viability and cell division?

I have trouble growing a cell line that I had just purchased from a cell line distributor. The cells I bought were produced in 2016 and when they were seedling they couldn't grow. There were some...

02 February 2021 3,751 5 View

Buffer for protein solubilization after acetone/AS precipitation that is compatible with cell stimulation?

Hello! I am precipitating some proteins (with either acetone or ammonium sulphate) and I need to resuspend the protein pellet in a buffer that properly keeps the proteins soluble AND which is...

27 January 2021 6,958 4 View

I'm looking for a dataset of eeg recorded during the pain stimulation. Anyone can help me to find it?

I'm looking for a dataset of eeg recorded during the pain stimulation (prefered the cold pressure pain stimulation). Anyone can help me to find it?

25 January 2021 301 2 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

How to analize platelet titration results?

Hi, I am working with TRAP-stimulated and unstimulated platelets in PRP Right now I am titrating the antibody CD62P but I have several questions about how to analyze my data to select the best...

13 January 2021 8,991 3 View