Can anyone recommend any research done on creating help systems for softwares and/or products?

We observe and use help all softwares/products, be it Microsoft Help Center or FAQs section or even Tutorials. Has anyone ever worked on creating a help ecosystem be it for one product or...

16 June 2019 7,643 1 View

Please suggest me a suitable solvent for both liquid isoprene rubber and pvp?

I want to dissolve liquid isoprene rubber 410 grade in pvpk30 polymer

09 June 2019 5,652 3 View

How to convert JAXA .h5 data to tif using python ?

I have Jaxa data of soil moisture level 3 from gportal with an extension of .h5 Variable available are GeoPhysical_Quantity and time_number. I wanted to extract soil moisture layer and convert it...

27 March 2019 1,008 1 View

Which statistical test for normality is a good choice for sample size greater than 50?

I am conducting a study to compare the two independent groups of deaf students based on some parameters. My sample size is greater than 50.

01 August 2018 3,636 5 View

I am looking for a scale to measure tolerance of ambiguity. Can someone please help?

The Multiple Stimulus Types Ambiguity Tolerance Scale-II (MSTAT-II) by McLain ( 2009 ) is most recent instrument developed as per my search. Any ideas how I can get this scale to carry out my...

16 December 2017 8,710 1 View

Does anyone know any freeware for determining size distribution using SAXS data?

26 June 2017 641 3 View

Can someone share a good protocol of sectioning and H& E for mice pancreas? I am facing problem in getting intact sections?

After sacrifice of animals, I am keeping pancreas in 4% paraformaldehyde for 1-2 weeks. Then for rehydration in 15% sucrose for 1 day before going for sectioning. I am using cryotome for making...

22 December 2016 8,984 7 View

No Clear band in DNA-EMSA.?

I have a protein whose function is to reduce transcription factors. To check whether my protein is active I have to do DNA-EMSA. I am using my purified protein to reduce TF from nuclear extract...

11 February 2016 3,942 5 View

How to express protein in picchia?

I have protein around 15kDa which i am unable to get in BL21 i tried many strains, but still no success. i also encounter with no induction though i got induced earlier. So can i get protein in...

09 December 2015 3,281 3 View

Any advice on late CT values of housekeeping genes?

i make cDNA of 1microgram RNA which is DNAse treated. while performing qPCR i am getting late CT values of the house keeping gene. i am unable to understand this because previously i use to get CT...

04 December 2015 6,320 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

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01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View