Currently nanoparticles and natural / artificial nanovesicles (e.g., exosomes and liposomes) are being employed to improve the cryopreservation of semen from different organisms. Liposomes / nanoliposomes, with their ingredients of phospholipids and lipid chains, can replace the damaged lipid skeletons of the frozen / thawed spermatozoa. This is while, research shows the treatment of spermatozoa with exosomes improved the efficiency of freezing procedures. Nevertheless, further in vivo and fertility studies are required to investigate the influence of exosome treatment on sperm functions. The concentration to be used depends on the source and concentration of spermatozoa.