Global change in protein expression?

Dear all, When I run my yeast total protein lysate extracted after 10 minute-treatment with my compound, I observe a global up regulation in proteins, even my loading control protein. I suspect...

03 January 2021 1,388 8 View

Tetracycline/doxcycline treatment?

Hi all, I am not sure whether or not this question was asked. I wonder can I induce expression of my protein with tetracycline or doxycyxline while seeding cells instead of one day after seeding...

05 November 2020 2,417 4 View

How to determine the overall heat transfer coefficient of a water tank?

Hello, Assume that we have a vertical water tank with a vertical flue gas pipe that is located right in the middle of it. Flue gas pipe is constantly supplied with a high temperature flue gas...

01 September 2018 9,652 1 View

How will the vapor pressure behave during condensation when it is in a mixture with noncondensable gases?

Hello, Let's assume a flue gas/water vapor mixture at 80 degrees C. According to the partial pressure of the water vapor in the flue gas mixture, the dew point temperature is 65 degrees C. If the...

30 July 2018 9,955 3 View

Calcium Phosphate Transfection Problem?

Hi, I am trying to transfect 293T cells using 2M CaCl2 and 2X HBS (50 mM HEPES, pH 7.07, 10 mM KCl, 12 mM dextrose, 280 mM NaCl, 1.5 mM Na2PO4) However, some plates, still have particles (probably...

23 March 2018 5,856 7 View

SiRNA Transfection to COLO205, SW620, HT29?

Hi, Is there anyone who has done siRNA Transfection in COLO205, SW620, HT29 especially using either RNAiMAX or Lipofectamine3000? Besides do you have any experience to transfect/transduce plasmid...

04 December 2017 2,369 3 View

Normalization for Immunoprecipitation?

07 June 2016 5,408 3 View

Having problem with mutant IDH (Isocitrate dehydrogenase) enzyme overexpressed cells?

Hi, I am having trouble with cells overexpressed mutant IDH enzyme. Problem is that it does not work. In other words, I chose some genes as a positive control from other papers and evaluated their...

02 March 2016 6,347 3 View

How can I protect RWPE-1 cells from any contamination?

15 July 2014 610 10 View

Do Thermophotovoltaic Cells need to be in the Infrared Region?

Current researches on TPV systems are on IR region. We assume, a diode works in UV. While the diode temperature is increasing by heating, Pmax is increasing; Is this cell belongs to...

01 January 1970 122 1 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View