Hello,
I am planning on designing an experiment with FISH imaging with a collaborator.
I want to research the sample preparation of breast cancer tumor tissues before I start anything.
Can you recommend me a protocol or literature sources to follow on.
Spatial transciptomics is higly new and I would love to hear hands on experience.
Best
These links may be of help
1)__ June 2021 paper in Nature / Nature Reviews - Genetics
Integrating single-cell and spatial transcriptomics to elucidate intercellular tissue dynamics
https://www.nature.com/articles/s41576-021-00370-8
2)__ 10-X Genomics
A company and not hands on information…
but a decent introduction to the ideas
of spatial transcriptomics
https://www.10xgenomics.com/spatial-transcriptomics
3)__ July 2021 paper in Science
Embryo-scale, single-cell spatial transcriptomics
Access to full paper by one of three science literature groups
or purchase of the full paper
https://science.sciencemag.org/content/373/6550/111
4)__ May 2021 paper in “Frontiers of Cell Biology”
Integrating Spatial Transcriptomics and Single-Cell RNA-seq Reveals the Gene Expression Profling of the Human Embryonic Liver
This is the full paper
https://www.frontiersin.org/articles/10.3389/fcell.2021.652408/full