Dear all,
May I know your 384-well plate poly-L-lysine coating protocol for HEK293 cells? Besides, How sensitive is poly-l-lysine to environment conditions such as leaving the stock at room temperature for awhile would immediately adversely affect the product efficiency? Thanks
Regards,
Dear Mert Burak!
Please You look at the article:
Article A High-throughput Calcium-flux Assay to Study NMDA-receptors...
3. Optimization of NMDAR Expression Levels
Prepare a 384-well plate with HEK293 cells (recommendation of 10,000 cells/well, 30 µL of media) and include the protection compounds as described in step 2.1.
Article A homogeneous microplate assay for agents that kill mammalia...
Cells All cells were cultured at 37 °C and 5% CO2. HMEC cells were purchased from Clonetics (Walkersville, MD). HMEC cells were maintained in DFCI-1 (32). HEK293 and HT29 cells were purchased from ATCC (Manassas, VA).
Hi Alexandr,
Thanks for your answer. Unfortunately, i couldnt find detail on how they coat the plates in that paper. They stated that they had used PDL.
I have PLL, but it should also work for HEK293.
Cheers,
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