What is the optimum concentration of the Sodium citrate Dihydrate to be chosen for IEC cation exchange purification?

More Amulya Ramakrishna's questions See All

How to measure Corporate Social responsibility?

My research objective is to find the impact of CSR on financial performance of the companies. How to measure the CSR as independent variable?

14 July 2024 2,846 5 View

Why is protein getting precipitated during dialysis?

I purified a protein containing His tag via Ni-NTA chromatography wherein the protein was eluted in elution buffer containing 500mM imidazole, 50 mM Tris, 500mM Nacl. Since my next step of...

31 March 2024 1,300 2 View

What are the best methods for measuring Corporate governance and corporate social responsibility?

In Indian context i am trying to verify the financial performance in relation to CG and CSR. Hence for empirical analysis this is required.

19 January 2024 5,593 6 View

Why should researchers review journal articles for free when access to journal articles is not free?

Often researchers get articles to review and they are paid nothing. The same publisher charge for accessing the journal articles. Doesn't it seems nonsense? Expecting your view in this regard.

01 October 2023 640 18 View

Could someone please inform me of the specifications for the lyophilizer that must be used to freeze-dry the clay samples??

I need clarity regarding the following parameters 1) Condenser Temperature ( Should I go for -50 degrees or -80 degrees) 2) Vacuum pressure to be applied

11 August 2023 8,762 1 View

Technique to study burst and pausing?

Hi, I am looking to study transcriptional burst and RNA Pol II pausing at the promoter of a gene. What are the best methods to study such events at a locus? Most of the papers I saw focus on high...

04 June 2023 9,289 0 View

Telomere length determination from human genomic DNA?

In order to assess the telomere length in human blood samples by qpcr, what is the best standard reference one can use for drawing the standard curve? I came across papers which use a pool of...

03 March 2023 2,324 7 View

Fluorescent dyes in ischemia models?

I am inducing ischemia by OGD in vitro. If I want to estimate mitochondrial membrane potential or look at ROS levels by means of fluorescent dyes, can I fix the cells and then stain for these? I...

17 January 2023 2,257 4 View

Similarity and Difference?

In Psychological Assessment and Testing, how the scale, test, battery, and inventory are similar and different from each other?

09 January 2023 3,764 5 View

What do F values represents (numbers in parenthesis)?

In, one of my manuscripts, i have mentioned [F (4, 19) = 119.3, P < 0.001]. The reviewer asked 'What do F values represent (numbers in parenthesis)?. Here 4 indicates the number of groups, i.e...

10 March 2022 8,225 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View