Amulya Ramakrishna

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Questions related from Amulya Ramakrishna

What is the optimum concentration of the Sodium citrate Dihydrate to be chosen for IEC cation exchange purification?

I am purifying a protein having pI of 6.63. So I am using Sodium citrate buffer (pH=5.5) for preparing wash buffer (Buffer A) and Elution buffer (Buffer B) for IEC. Kindly let me know what is the...

05 July 2024 2,775 2 View

Why is protein getting precipitated during dialysis?

I purified a protein containing His tag via Ni-NTA chromatography wherein the protein was eluted in elution buffer containing 500mM imidazole, 50 mM Tris, 500mM Nacl. Since my next step of...

01 April 2024 1,339 2 View